February 5th, 2016
In Attendance:
- Lindsey Cabanas
- Naina Iyengar
- Sindhuja Kuchibhatla
Accomplished Tasks:
- Devised a protocol for the first verification activity
Future Tasks:
- Get protocol revised by Dr. Hall if there is more information needed to be added
February 2nd, 2016
In Attendance:
- Lindsey Cabanas
- Naina Iyengar
- Sindhuja Kuchibhatla
- Dr. Hall
Accomplished Tasks:
- Moved chemicals to Dr. Hall’s lab (Bio 218)
- Lab safety procedures addressed
- Practiced using spectrophotometers with sample solutions
- Everyone familiarized themselves with the functions of the spectrophotometer and transferring chemicals into and out of the cuvettes
- Practiced measuring absorbances with different concentrations of sample solutions
- Recorded sample data obtained from the spectrophotometers to get a standard curve
Future Tasks:
- Naina: figure out what size needle is needed to fill amylase flask with nitrogen gas
- All: continue working on NEBEC paper and IRB
January 29th, 2016
In Attendance:
- Lindsey Cabanas
- Naina Iyengar
- Sindhuja Kuchibhatla
- Dr. Hall
Accomplished Tasks:
- Rough protocol written out for the procedure of creating mock saliva
- Safety hazards addressed when working in the lab
Future Tasks:
- Naina:
- Call Sigma to figure out how to store chemicals under inert gas
- All:
- Work on exact procedure for the chemicals kit
January 27th, 2016
In Attendance:
- Lindsey Cabanas
- Naina Iyengar
- Sindhuja Kuchibhatla
Accomplished Tasks:
- Process Order Forms for remaining materials needed
- All mechanical parts ordered
- Remaining electrical parts ordered
- Quantity of saliva obtained for mock saliva modified to fit the amount of PBS available
- IRB template created & started
- NEBEC template created & started
Future Tasks:
- Learn Safety Protocol to work in Dr. Hall’s lab
- Start optimizing volumes on Wednesday
November 9th, 2015
Accomplished Tasks:
- Safety survey completed
- Selection of alpha-amylase sample
- Light sensor changed from photo-resistor to TSL2561 sensor
- Talked to Joe about materials for casing and holders
- 3D printing considered for holders
- Blackbox material considered for casing
Future Tasks:
- Order electronic devices and chemical reagents
- Continue working on drawings for casing and holders
November 2nd, 2015
Accomplished Tasks:
- Materials for casing considered
- Arduino Uno selected for micro-controller
- LED holder, cuvette holder, light sensor holder needed and materials considered for each
- Protocol for converting absorbances to severity level determined
- Choosing between two types of alpha-amylase samples to be used for testing
Future Tasks:
- Start drawings for holders and casing
- Determine sensitivity of available mass scales for verification/validation
- Determine final list of reagents including specific alpha-amylase sample
October 27th, 2015
Accomplished Tasks:
- Use of micro-controller to correlate absorbance measurement to biomarker concentration
- Photo-resistor chosen for light sensor
Future Tasks:
- Determine verification and validation activities for each device requirement
October 19th, 2015
Accomplished Tasks:
- Alpha-amylase chosen as biomarker
- Photodiode, photo-resistor, other light sensors found for colorimetric detection
- Kinetics of alpha-amylase reaction to produce color change found
- Casing to encompass darkness considered
Future Tasks:
- Determine specific light sensor to be used by considering advantages and/or disadvantages of each
- How will the absorbance measurement be converted to concentration of biomarker
October 5th, 2015
Accomplished Tasks:
- Correlation of alpha-amylase and LDH to periodontal disease activity found
- Colorimetric sensor needed
Future Tasks:
- Choose one biomarker
- Casing for the device
- Sensitivity of sensor
September 28th, 2015
Accomplished Tasks:
- Biomarkers: alpha-amylase, LDH, and pH considered
- Optical sensor needed
Future Tasks:
- Narrow down biomarker selection
- Consider types of optical sensors needed
- Design process
September 21st, 2015
Accomplished Tasks:
- Biomarker detection needed
Future Tasks:
- Determine what kind of biomarker
- How does the biomarker correlate to disease activity
- How are the reagents going to be delivered